fluorescent ros indicator cm-h2dcfda reagent Search Results


dichlorodihydrofluorescein diacetate  (Thermo Fisher)
97
Thermo Fisher dichlorodihydrofluorescein diacetate
Dichlorodihydrofluorescein Diacetate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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fluorescent probe 5 6 chloromethyl 2 7 dichlorofluorescein diacetate  (Thermo Fisher)
99
Thermo Fisher fluorescent probe 5 6 chloromethyl 2 7 dichlorofluorescein diacetate
Fluorescent Probe 5 6 Chloromethyl 2 7 Dichlorofluorescein Diacetate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ros+indicator+cm-h2dcfda+reagent/2'%2C7'-Dichlorofluorescein/pm30658075-67-7-14
Average 99 stars, based on 1 article reviews
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fluorescent probe 5  (MedChemExpress)
94
MedChemExpress fluorescent probe 5
Fluorescent Probe 5, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
fluorescent probe 5 - by Bioz Stars, 2026-11
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cm h2dcfda in pbs  (Thermo Fisher)
99
Thermo Fisher cm h2dcfda in pbs
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
Cm H2dcfda In Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ros+indicator+cm-h2dcfda+reagent/PBS/pmc11102918-314-24-27
Average 99 stars, based on 1 article reviews
cm h2dcfda in pbs - by Bioz Stars, 2026-11
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dichlorodihydro fluorescein diacetate  (Thermo Fisher)
99
Thermo Fisher dichlorodihydro fluorescein diacetate
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
Dichlorodihydro Fluorescein Diacetate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ros+indicator+cm-h2dcfda+reagent/Fluorescein/pm15928461-79-17-20
Average 99 stars, based on 1 article reviews
dichlorodihydro fluorescein diacetate - by Bioz Stars, 2026-11
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accuri c6 plus flow cytometer  (Becton Dickinson)
90
Becton Dickinson accuri c6 plus flow cytometer
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
Accuri C6 Plus Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
accuri c6 plus flow cytometer - by Bioz Stars, 2026-11
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ezs-fl fluorescent plate reader  (Asahi Techno Glass)
90
Asahi Techno Glass ezs-fl fluorescent plate reader
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
Ezs Fl Fluorescent Plate Reader, supplied by Asahi Techno Glass, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ros+indicator+cm-h2dcfda+reagent/ezs+fl+fluorescent+plate+reader/10__1007_slash_bf03402010-42-22-26
Average 90 stars, based on 1 article reviews
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acetyl ester  (Thermo Fisher)
95
Thermo Fisher acetyl ester
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
Acetyl Ester, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ros+indicator+cm-h2dcfda+reagent/Dihydrofluorescein+diacetate%2C+97%25/pm26239821-61-27-29
Average 95 stars, based on 1 article reviews
acetyl ester - by Bioz Stars, 2026-11
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spectra max plus microplate reader  (Molecular Devices LLC)
99
Molecular Devices LLC spectra max plus microplate reader
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
Spectra Max Plus Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ros+indicator+cm-h2dcfda+reagent/SpectraMax+ABS+PLUS+Microplate+Reader/pm23876501-74-19-24
Average 99 stars, based on 1 article reviews
spectra max plus microplate reader - by Bioz Stars, 2026-11
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inverted fluorescence microscope  (Nikon)
99
Nikon inverted fluorescence microscope
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
Inverted Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
inverted fluorescence microscope - by Bioz Stars, 2026-11
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6 chloromethyl 20 70dichlorodihydrofluorescein diacetate  (Thermo Fisher)
99
Thermo Fisher 6 chloromethyl 20 70dichlorodihydrofluorescein diacetate
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
6 Chloromethyl 20 70dichlorodihydrofluorescein Diacetate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
6 chloromethyl 20 70dichlorodihydrofluorescein diacetate - by Bioz Stars, 2026-11
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dyes  (Thermo Fisher)
99
Thermo Fisher dyes
a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).
Dyes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ros+indicator+cm-h2dcfda+reagent/FURA+2-AM/pm22886740-45-10-19
Average 99 stars, based on 1 article reviews
dyes - by Bioz Stars, 2026-11
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Image Search Results


a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).

Journal: Cell Discovery

Article Title: DEPDC5 protects CD8 + T cells from ferroptosis by limiting mTORC1-mediated purine catabolism

doi: 10.1038/s41421-024-00682-z

Figure Lengend Snippet: a Representative histograms showing phosphorylated S6-S235/236 levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice after treatment with vehicle only or rapamycin (100 μg/kg daily for 4 weeks). After treatment, splenocytes were isolated and stained with a fixable viability dye, anti-mouse CD8 antibody, and anti-S6-S235/236 phosphorylation antibody for analysis by flow cytometry. b Summary bar graph showing MFI of phosphorylated S6-S235/236 in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice ( n = 3) as described as a . c – e Representative flow cytometry plots ( c ) and summary bar graphs showing percentage of CD8 + T cells ( d ) and CD4 + T cells ( e ) in LN from Depdc5 ncl and Depdc5 tko mice treated with rapamycin or vehicle only as in a . LN single cell suspensions were prepared from Depdc5 ncl and Depdc5 tko mice treated daily with vehicle only or 100 μg/kg rapamycin for 4 weeks prior to staining with a fixable viability dye and antibodies against CD4 and CD8 prior to flow cytometry analysis. f, g Representative histogram ( f ) and a summary bar graph ( g ) showing relative lipid ROS levels in splenic CD8 + T cells from Depdc5 ncl and Depdc5 tko mice treated with vehicle only or rapamycin for 4 weeks. Splenocytes from the same mice in a were cultured in the presence of 2 μM BODIPY™ 581/591 C11 for 4 h, washed twice in PBS, then stained with a fixable viability dye and antibodies against CD4 and CD8 for flow cytometry analysis. Error bars indicate means ± SEM. (NS, not significant, ** P < 0.01 by unpaired Student’s t -test).

Article Snippet: Mouse splenocytes were seeded in a Round 96-well plate with 10 μM allopurinol treated for 2 h or not, then stained with 5 μM CM-H2DCFDA in PBS (Life Technologies) at 37 °C for 15 min. After incubation, the cells were collected by centrifuge at 1500 rpm for 5 min at 4 °C, followed by cold PBS wash twice and staining surface marker; ROS emission was recorded on FITC panel fluorescence levels by flow cytometry.

Techniques: Isolation, Staining, Phospho-proteomics, Flow Cytometry, Cell Culture